ISOLASI DAN KARAKTERISASI FISIOLOGIS PGPR DARI RIZOFER TANAMAN KACANG TANAH (Arachis hypogaea) VARIETAS LOKAL MAYBRAT
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Jul 18, 2023
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Abstract
Sustainable agriculture systems utilizing PGPR (Plant Growth-Promoting Rhizobacteria) have the potential to increase due to their environmentally friendly and practical nature, to replace the increasing use of chemical-based fertilizers and pesticides. Peanut (Arachis hypogea L) local variety Maybrat is a plant that has the potential to be isolated by bacteria in its rhizosphere area to be used as a biostimulant candidate. This study aimed to determine the type of rhizobacteria in the rhizosphere area associated with local varieties of peanuts in the Maybrat Regency and obtain superior rhizobacteria for biostimulants (PGPR). A total of 8 isolates were successfully isolated from peanut rhizosphere and based on physiological characteristics showed that KM2 isolates produced the highest IAA of 2,625 mg l-1, and KM8 isolates produced the highest GA3 concentration of 4.19 mg l-1. KM7 isolates produce salicylates with the highest concentration of 0.788 mg l-1, while KM3 isolates produce the highest type of catechols of 1.89 mg l-1. The KM8 isolate is capable of dissolving the highest phosphate of 12.46 mg l-1. Cyanide production tests showed that all eight bacterial isolates did not have the ability to produce cyanide. Based on physiological characteristics, it shows that these 8 isolates have the potential to be biostimulants and biofertilizers for plants.
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Patty, K., Huwae, L., & Mayor, Y. (2023). ISOLASI DAN KARAKTERISASI FISIOLOGIS PGPR DARI RIZOFER TANAMAN KACANG TANAH (Arachis hypogaea) VARIETAS LOKAL MAYBRAT. SOSCIED, 6(1), 234-242. https://doi.org/10.32531/jsoscied.v6i1.642
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
LPPM Politeknik Katolik Saint Paul Sorong
References
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Grobelak, A., Napora, A., Kacprzak, M., 2015. Using plant growth-promoting rhizobacteria (PGPR) to improve plant growth. Ecological Engineering. 84: 22-28. Doi: 10.1016/j.ecoleng.2015.07.019
Mu’minah., Baharudin., Subair, H., Faharudin., 2015. Isolation and Screening Bacterial Exopolysaccarida from Potato Rhizospere in Highland and The Potential as a Producer Indole Acetic Acid (IAA). Procedia Food Science. 3: 74-81.
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Sgroy V, Cassan F, Masciarelli O, Del Papa MF, Lagares A, Luna V. Isolation and characterization of endophytic plant growth-promoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associated to the halophyte Prosopisstrombulifera. Appl Microbiol Biotechnol 2009:85371–81.
Masciarelli, O., Llanes, A., Luna, V., 2013. A New PGPR Co-Inoculated With Bradyrhizobium japonicum Enhances Soybean Nodulation. Microbiological Research. 169: 609-615
[
Rajkumar, M., Bruno, L.B., Banu, J.R., 2017. Alleviation of environmental stress in plants: The role of beneficial Pseudomonas spp. Crit. Rev. Environ. Sci. Technol. 47 (6), 372–407. https://doi.org/10.1080/10643389.2017.1318619.
Arzanesh, M.H., Alikhani, H.A., Khavazi, K., Rahimian, H.A., Miransari, M., 2011. Wheat (Triticum aestivum L.) Growth Enhancement by Azospirillum sp. Under Drought Stress. World J. Microbiol. Biotechnol. 27 (2), 197–205. https://doi.org/10.1007/s11274-010-0444-1.
Ali, S.Z., Sandhya, V., Venkateswar Rao, L., 2014. Isolation and characterization of drought-tolerant ACC deaminase and exopolysaccharide-producing fluorescent Pseudomonas sp. Ann. Microbiol. 64 (2), 493–502. https://doi.org/10.1007/s13213-013-0680-3
Goswami, D., Vaghela, H., Parmar, S., Dhandhukia, P., Thakker, N. J., 2013. Plant Growth Promoting Potential of Pseudomonas spp. Strain OG Isolated From Marine Water. Plant Interaction. 8: 281-290. https://doi.org/10.1080/17429145.2013.768360
Jan AT, Azam M, Ali A, Haq Q. Novel Approaches of Beneficial Pseudomonas in Mitigation of Plant Diseases – an Appraisal. J Plant Interact 2011;6(4):195–205.
Hayat R, Ali S, Amara U, Khalid R, Ahmed I. Soil beneficial bacteria and their role inplant growth promotion: a review. Ann Microbiol 2010;60(4):579–98.
Backer, R., Rokem. S.J., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S., Smith, L.D., 2018. Plant Growth-Promoting Rhizobacteria: Contex, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science. 9: 1473. https://doi.org/10.3389/fpls.2018.01473
Delaplace, P., Delory, B. M., Baudson, C., Mendaluk-Saunier, De Cazenave, M., Spaepen, S., et al. (2015). Influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass Brachypodium distachyon (L.) P. Beauv. BMC Plant Biol. 15:195. doi: 10.1186/s12870-015-0585-3
Poli, A., Lazzari, A., Prigione, V., Voyron, S., Spadaro, D., and Varese, G. C. (2016). Influence of plant genotype on the cultivable fungi associated to tomato rhizosphere and roots in different soils. Fungal Biol. 120, 862–872. doi: 10.1016/j.funbio.2016.03.008
Wintermans, P. C., Bakker, P. A., and Pieterse, C. M. (2016). Natural genetic variation in Arabidopsis for responsiveness to plant growth-promoting rhizobacteria. Plant Mol. Biol. 90, 623–634. doi: 10.1007/s11103-016-0442-2
Riaz, U., Murtaza, G., Anum, W., Samreen, T., Sarfraz, M., Nazir, Z.M., 2020. Plant Growth-Promoting Rhizobacteria (PGPR) as Biofertilizers and Biopesticides. Springer. doi: 10.1007/978-3-030-48771-3_11
Kalayanasudaram, T.G., Syed, N., Subburamu, K., 2021. Recent Development in plant growth promoting rhizobacteria (PGPR) for sustainable agriculture. Elsevier. doi: 10.1016/B978-0-12-821406-0.00017-5
Carsten, S.J., Mathis, H.H., 2014. Agricultural soil, pesticides and microbial diversity. Curr. Opin. Biotechnol. 27, 15-20
Pandey, A., Tripathi, A., Srivastava, P., Choudhary, K.K., Dikshit, A., 2019. Plant growth promoting microorganisms in sustainable agriculture. Elsevier. doi: 10.1016/B978-0-12-817004-5.00001-4
Riaz, U., Murtaza, G., Anum, W., Samreen, T., Sarfraz, M., Nazir, Z.M., 2020. Plant Growth-Promoting Rhizobacteria (PGPR) as Biofertilizers and Biopesticides. Springer. doi: 10.1007/978-3-030-48771-3_11
Kesaulya, H., Baharudin., Zakaria, B., Syaiful, A.S., 2015. Isolation and physiological characterization of PGPR from potato plant rhizosphere in medium land of Buru Island. Procedia Food Science. 3: 190-199. doi: 10.1016/j.profoo.2015.01.021
Grobelak, A., Napora, A., Kacprzak, M., 2015. Using plant growth-promoting rhizobacteria (PGPR) to improve plant growth. Ecological Engineering. 84: 22-28. Doi: 10.1016/j.ecoleng.2015.07.019
Mu’minah., Baharudin., Subair, H., Faharudin., 2015. Isolation and Screening Bacterial Exopolysaccarida from Potato Rhizospere in Highland and The Potential as a Producer Indole Acetic Acid (IAA). Procedia Food Science. 3: 74-81.
Pham, T., Bui, D. X., Trang, K.V.L., Le, T., Nguyen, L.M., Trinh, D., Phoung, D.T.N., Khoo, S.K., Chew, W.K., Show, L.P., 2022. Isolation of indole-3-acetic acid-producing Azospirillum brasilense from Vietnamese wet rice: Co-immobilization of isolate and microalgae as a sustainable biorefinery. Journal Of Biotechnology. 349: 12-20. doi.org/10.1016/j.jbiotec.2022.03.007
Gutiérrez-Ma˜nero F, Ramos-Solano B, Probanza A, Mehouachi J, Tadeo F, TalonM. The plant-growth-promoting rhizobacteria Bacillus pumilus and Bacilluslicheniformis produce high amounts of physiologically active gibberellins.Physiol Plantarum 2001;111:206–11
Sgroy V, Cassan F, Masciarelli O, Del Papa MF, Lagares A, Luna V. Isolation and characterization of endophytic plant growth-promoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associated to the halophyte Prosopisstrombulifera. Appl Microbiol Biotechnol 2009:85371–81.
Masciarelli, O., Llanes, A., Luna, V., 2013. A New PGPR Co-Inoculated With Bradyrhizobium japonicum Enhances Soybean Nodulation. Microbiological Research. 169: 609-615
[
Rajkumar, M., Bruno, L.B., Banu, J.R., 2017. Alleviation of environmental stress in plants: The role of beneficial Pseudomonas spp. Crit. Rev. Environ. Sci. Technol. 47 (6), 372–407. https://doi.org/10.1080/10643389.2017.1318619.
Arzanesh, M.H., Alikhani, H.A., Khavazi, K., Rahimian, H.A., Miransari, M., 2011. Wheat (Triticum aestivum L.) Growth Enhancement by Azospirillum sp. Under Drought Stress. World J. Microbiol. Biotechnol. 27 (2), 197–205. https://doi.org/10.1007/s11274-010-0444-1.
Ali, S.Z., Sandhya, V., Venkateswar Rao, L., 2014. Isolation and characterization of drought-tolerant ACC deaminase and exopolysaccharide-producing fluorescent Pseudomonas sp. Ann. Microbiol. 64 (2), 493–502. https://doi.org/10.1007/s13213-013-0680-3
Goswami, D., Vaghela, H., Parmar, S., Dhandhukia, P., Thakker, N. J., 2013. Plant Growth Promoting Potential of Pseudomonas spp. Strain OG Isolated From Marine Water. Plant Interaction. 8: 281-290. https://doi.org/10.1080/17429145.2013.768360
Jan AT, Azam M, Ali A, Haq Q. Novel Approaches of Beneficial Pseudomonas in Mitigation of Plant Diseases – an Appraisal. J Plant Interact 2011;6(4):195–205.
Hayat R, Ali S, Amara U, Khalid R, Ahmed I. Soil beneficial bacteria and their role inplant growth promotion: a review. Ann Microbiol 2010;60(4):579–98.