SUKSESI BAKTERI PADA FERMENTASI KULIT BUAH NANAS (Ananas comosus (L.) Merr)
Article Sidebar
Published
Nov 27, 2024
Main Article Content
Abstract
The utilization of pineapple peel in the fermentation process produces bacteria and probiotics that are beneficial for human health. The fermentation process is carried out to enhance the nutrition, shelf life, and organoleptic properties of food products. The presence of microbes, especially bacteria, is believed to play an important role in the fermentation process of pineapple peel. However, the specific bacteria involved in the fermentation of pineapple peel are not yet fully known. The aim of this research is to study the bacterial succession during the fermentation of pineapple peel (Ananas comosus (L.) Merr) and to identify the types of bacteria obtained during the fermentation process. This research uses a qualitative descriptive laboratory-based method by conducting morphological identification of bacteria and biochemical testing to determine the types of bacteria present in the fermentation of pineapple peel. Based on the identification data, 11 bacterial isolates were found during the fermentation of pineapple peel. The isolates were identified macroscopically and then subjected to a series of biochemical tests. The results of the research indicate that the microorganisms involved in the fermentation of pineapple peel belong to the genera Staphylococcus sp., Bacillus sp., and Lactobacillus sp.. The microbial succession during pineapple peel fermentation shows that the bacterial population of the genus Staphylococcus sp. dominates on day 0 of fermentation, while the genus Bacillus sp. undergoes a succession pattern, experiencing growth on day 1 and continuing to increase until day 3. Meanwhile, bacteria from the genus Lactobacillus sp. only appeared on day 1 of fermentation.
Article Details
How to Cite
Pelealu, I., Lawalata, H., Tengker, A., & Posumah, D. (2024). SUKSESI BAKTERI PADA FERMENTASI KULIT BUAH NANAS (Ananas comosus (L.) Merr). SOSCIED, 7(2), 629-640. https://doi.org/10.32531/jsoscied.v7i2.861
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
LPPM Politeknik Katolik Saint Paul Sorong
References
Wahyuni, S. A., Kadarusno, A. H., & Suwerda, B. (2016). Pemanfaatan Saccharomyces Cereviceae Dan Limbah Buah Nanas Pasar Beringharjo Yogyakarta Untuk Pembuatan Bioetanol. Sanitasi: Jurnal Kesehatan Lingkungan, 7(4), 151-159.
Erukainure, O.L., J.A. Ajiboye, R.O. Adejobi, O.Y. Okafor, S.O. Adenekan. 2011. Protective Effect Of Pineapple (Ananas Comosus) Peel Extract On Alcohol- Induced Oxidative Stress In Brain Tissues Of Male Albino Rats. Asian Pac. J. Trop. Disease. 5-9.
Punbasayakul, N., Samart, K., & Sudmee, W. (2018, January). Antimicrobial Activity Of Pineapple Peel Extract. In Proceeding Of Innovation Of Functional Foods In Asia Conference.
Suerni E, Alwi M, Guli MM. 2013. Uji Daya Hambat Ekstrak Kulit Nanas (Ananas Comosus L. Merr), Salak (Salacca Eduilis Reinw.) Dan Mangga Kweni (Mangifera Odorata Griff.) Terhadap Daya Hambat Staphylococcus Aureus. Biocelebes. 7(1):1-8.
Prihatman, K. (2000). Nanas (Ananas Comosus). TTG Budidaya Pertanian. Jakarta, 17.
Suprihatin, D. S. P. (2010). Pembuatan Asam Laktat Dari Limbah Kubis. Makalah SEMNAS Ketahanan Pangan Dan Energi, Teknik Kimia Soebardjo Brotohartandjono, Surabaya.
Prabowo, A. (2011). Pengawetan Dedak Padi Dengan Cara Fermentasi. URL: Http://Sumsl. Litbang. Deptan. Go. Id/Index. Php/Component/Content/Article/53-It-1/206-Dedak-Padi, 6.
Suhartini, S., Hidayat, N., & Rosaliana, E. (2013). Influence Of Powdered Moringa Oleifera Seeds And Natural Filter Media On The Characteristics Of Tapioca Starch Wastewater. International Journal Of Recycling Of Organic Waste In Agriculture, 2, 1-11.
Tortora G.J., Funke B.R., Case C.L. 2010. Microbiology : An Introduction. 10th Ed. California : Benjamin & Cummings.
[
Boleng, D. T. (2015). Bakteriologi Konsep-Konsep Dasar.
Hadioetomo R. S. 1993. Mikrobiologi Dasar Dalam Praktek. PT. Gramedia, Jakarta.
Kosasi, C., Lolo, W. A., & Sudewi, S. (2019). Isolasi Dan Uji Aktivitas Antibakteri Dari Bakteri Yang Berasosiasi Dengan Alga Turbinaria Ornata (Turner) J. Agardh Serta Identifikasi Secara Biokimia. Pharmacon, 8(2), 351-359.
Ummamie, L., Rastina, R., & Erina, E. (2017). Isolasi Dan Identifikasi Escherichia Coli Dan Staphylococcus Aureus Pada Keumamah Di Pasar Tradisional Lambaro, Aceh Besar. Jurnal Ilmiah Mahasiswa Veteriner, 1(3), 574-583.
Ulfa, M., Khairi, N., & Maryam, F. (2016). Formulasi Dan Evaluasi Fisik Krim Body Scrub Dari Ekstrak Teh Hitam (Camellia Sinensis), Variasi Konsentrasi Emulgator Span-Tween 60. Jurnal Farmasi UIN Alauddin Makassar, 4(4), 179-185.
Widowati, R., Handayani, S., & Lasdi, I. (2019). Aktivitas Antibakteri Minyak Nilam (Pogostemon Cablin) Terhadap Beberapa Spesies Bakteri Uji. Jurnal Pro-Life, 6(3), 237-249.
Lawalata, H. J., Rengkuan, M. D., & Howan, D. H. O. (2018). Identifikasi Fenotipik Dan Genotipik Bakteri Asam Laktat Penghasil Antibakteri Dari Buah Langsat (Lansuium Domesticum) Matang.
Lawalata, H.J Dan Satiman, U. 2015. Identification Of Lactic Acid Bacteria Proteolytic Isolated From An Indonesian Traditional Fermented Fish Sauce Bakasang By Amplified Ribosomal DNA Restriction Analysis (ARDRA). Int. Journal Of Chemtech Research. 8 (12) Pp 630-636.
Zahid, A. H., Han, Q., Jia, X., Li, S., Hangjia, H., & Liu, H. (2020). Highly Stable 3D Multilayered Nanoparticles-Based Β-Bi2O3 Hierarchitecture With Enhanced Photocatalytic Activity. Optical Materials, 109, 110389.
Fitrah, I. D. (2013). Isolasi Pasteurella Multocida Pada Kuda Dan Sensitivitasnya Terhadap Antibiotik. Jurnal Medika Veterinaria, 7(2).
Holt, R. I. (2004). Diagnosis, Epidemiology And Pathogenesis Of Diabetes Mellitus: An Update For Psychiatrists. The British Journal Of Psychiatry, 184(S47), S55-S63.
Nur, H. S. (2010). Suksesi Mikroba Dan Aspek Biokimiawi Fermentasi Mandai Dengan Kadar Garam Rendah. Makara Journal Of Science, 13(1), 3.
Fitri, L., & Yasmin, Y. (2011). Isolasi Dan Pengamatan Morfologi Koloni Bakteri Kitinolitik. Jurnal Ilmiah Pendidikan Biologi, 3(2), 20-25.
Cappuccino Dan Sherman, 2013:73-74).
Haryani, Y., Chainulfiffah., Rustiana. 2012. Fermentasi Karbohidrat Oleh Isolat Salmonella Sp. Dari Jajanan Pinggir Jalan. Jurnal Ind.Che.Acta. ISSN 2085-0050
Lawalata, H. J., Kumajas, J., Tengker, S. M., Runtuwene, K. M., Hasani, R. S., & Weken, M. M. (2023). Lactic Acid Bacteria as an Exopolysaccharides (EPS) Producing Starter from Pakoba Fruit (Syzygium sp.), Endemic Species at Minahasa, North Sulawesi. Journal of Pure & Applied Microbiology, 17(4).
Erukainure, O.L., J.A. Ajiboye, R.O. Adejobi, O.Y. Okafor, S.O. Adenekan. 2011. Protective Effect Of Pineapple (Ananas Comosus) Peel Extract On Alcohol- Induced Oxidative Stress In Brain Tissues Of Male Albino Rats. Asian Pac. J. Trop. Disease. 5-9.
Punbasayakul, N., Samart, K., & Sudmee, W. (2018, January). Antimicrobial Activity Of Pineapple Peel Extract. In Proceeding Of Innovation Of Functional Foods In Asia Conference.
Suerni E, Alwi M, Guli MM. 2013. Uji Daya Hambat Ekstrak Kulit Nanas (Ananas Comosus L. Merr), Salak (Salacca Eduilis Reinw.) Dan Mangga Kweni (Mangifera Odorata Griff.) Terhadap Daya Hambat Staphylococcus Aureus. Biocelebes. 7(1):1-8.
Prihatman, K. (2000). Nanas (Ananas Comosus). TTG Budidaya Pertanian. Jakarta, 17.
Suprihatin, D. S. P. (2010). Pembuatan Asam Laktat Dari Limbah Kubis. Makalah SEMNAS Ketahanan Pangan Dan Energi, Teknik Kimia Soebardjo Brotohartandjono, Surabaya.
Prabowo, A. (2011). Pengawetan Dedak Padi Dengan Cara Fermentasi. URL: Http://Sumsl. Litbang. Deptan. Go. Id/Index. Php/Component/Content/Article/53-It-1/206-Dedak-Padi, 6.
Suhartini, S., Hidayat, N., & Rosaliana, E. (2013). Influence Of Powdered Moringa Oleifera Seeds And Natural Filter Media On The Characteristics Of Tapioca Starch Wastewater. International Journal Of Recycling Of Organic Waste In Agriculture, 2, 1-11.
Tortora G.J., Funke B.R., Case C.L. 2010. Microbiology : An Introduction. 10th Ed. California : Benjamin & Cummings.
[
Boleng, D. T. (2015). Bakteriologi Konsep-Konsep Dasar.
Hadioetomo R. S. 1993. Mikrobiologi Dasar Dalam Praktek. PT. Gramedia, Jakarta.
Kosasi, C., Lolo, W. A., & Sudewi, S. (2019). Isolasi Dan Uji Aktivitas Antibakteri Dari Bakteri Yang Berasosiasi Dengan Alga Turbinaria Ornata (Turner) J. Agardh Serta Identifikasi Secara Biokimia. Pharmacon, 8(2), 351-359.
Ummamie, L., Rastina, R., & Erina, E. (2017). Isolasi Dan Identifikasi Escherichia Coli Dan Staphylococcus Aureus Pada Keumamah Di Pasar Tradisional Lambaro, Aceh Besar. Jurnal Ilmiah Mahasiswa Veteriner, 1(3), 574-583.
Ulfa, M., Khairi, N., & Maryam, F. (2016). Formulasi Dan Evaluasi Fisik Krim Body Scrub Dari Ekstrak Teh Hitam (Camellia Sinensis), Variasi Konsentrasi Emulgator Span-Tween 60. Jurnal Farmasi UIN Alauddin Makassar, 4(4), 179-185.
Widowati, R., Handayani, S., & Lasdi, I. (2019). Aktivitas Antibakteri Minyak Nilam (Pogostemon Cablin) Terhadap Beberapa Spesies Bakteri Uji. Jurnal Pro-Life, 6(3), 237-249.
Lawalata, H. J., Rengkuan, M. D., & Howan, D. H. O. (2018). Identifikasi Fenotipik Dan Genotipik Bakteri Asam Laktat Penghasil Antibakteri Dari Buah Langsat (Lansuium Domesticum) Matang.
Lawalata, H.J Dan Satiman, U. 2015. Identification Of Lactic Acid Bacteria Proteolytic Isolated From An Indonesian Traditional Fermented Fish Sauce Bakasang By Amplified Ribosomal DNA Restriction Analysis (ARDRA). Int. Journal Of Chemtech Research. 8 (12) Pp 630-636.
Zahid, A. H., Han, Q., Jia, X., Li, S., Hangjia, H., & Liu, H. (2020). Highly Stable 3D Multilayered Nanoparticles-Based Β-Bi2O3 Hierarchitecture With Enhanced Photocatalytic Activity. Optical Materials, 109, 110389.
Fitrah, I. D. (2013). Isolasi Pasteurella Multocida Pada Kuda Dan Sensitivitasnya Terhadap Antibiotik. Jurnal Medika Veterinaria, 7(2).
Holt, R. I. (2004). Diagnosis, Epidemiology And Pathogenesis Of Diabetes Mellitus: An Update For Psychiatrists. The British Journal Of Psychiatry, 184(S47), S55-S63.
Nur, H. S. (2010). Suksesi Mikroba Dan Aspek Biokimiawi Fermentasi Mandai Dengan Kadar Garam Rendah. Makara Journal Of Science, 13(1), 3.
Fitri, L., & Yasmin, Y. (2011). Isolasi Dan Pengamatan Morfologi Koloni Bakteri Kitinolitik. Jurnal Ilmiah Pendidikan Biologi, 3(2), 20-25.
Cappuccino Dan Sherman, 2013:73-74).
Haryani, Y., Chainulfiffah., Rustiana. 2012. Fermentasi Karbohidrat Oleh Isolat Salmonella Sp. Dari Jajanan Pinggir Jalan. Jurnal Ind.Che.Acta. ISSN 2085-0050
Lawalata, H. J., Kumajas, J., Tengker, S. M., Runtuwene, K. M., Hasani, R. S., & Weken, M. M. (2023). Lactic Acid Bacteria as an Exopolysaccharides (EPS) Producing Starter from Pakoba Fruit (Syzygium sp.), Endemic Species at Minahasa, North Sulawesi. Journal of Pure & Applied Microbiology, 17(4).